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polY1 [2018-08-12 18:49:13]
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polY1 [2018-08-12 18:49:13]

translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci
locus
BSU23870
pI
9.95
mw
46.86 kDa
protein length
414 aa Sequence Blast
gene length
1242 bp Sequence Blast
function
generation of mutations in stationary phase
product
translesion synthesis (TLS-) DNA polymerase Y1
essential
no
ec
2.7.7.7
synonyms
yqjH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    2,482,269 → 2,483,513

    Phenotypes of a mutant

  • reduced UV-induced mutagenesis [Pubmed|12644484]
  • sensitive to blue light-induced DNA damage [pubmed|30054368]

    • The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

    • Protein family

  • DNA polymerase type-Y family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|PolY2], [protein|BE8D5E038C0FD748C0F9C3F18DCA601BFFE8EA52|UvrX]

    • Structure

  • [PDB|4IRC] (35% identity)

    • [SW|Localization]

  • cytoplasm (homogeneous)

    • Expression and Regulation

    Operons

    genes
    [gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]-[gene|61B7C51EB7E74226890010B61D8E41C02189A453|gndA]
    description
    [pubmed|22383849]

    regulation

  • constitutively expressed [Pubmed|15469515]
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    Biological materials

    Mutant

  • MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]
  • GP1111 (spc), available in [SW|Stülke] lab
  • BKE23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
  • BKK23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG

    • References

    Reviews

  • 22933559,29856930

    • Original publications

  • 12644484,15469515,16045613,19924481,23686288,25713353,30054368,30096406